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Cloning and Expression
Enzymes
MscI
Supplier Images
MscI
MscI from New England Biolabs
Be the first to write a review!
Citations:
(8)
Supplier Page
Supplier Page from
New England Biolabs for
MscI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MscI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0534L
Concentration
3-15 u/µL
Quantity
1250 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
TGG/CCA
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (8)
(1)
Engineering yeast to induce the synthesis of GPI-APs with a permanent phosphoethanolamine on mannose 2 of the glycan moiety
STAR Protocols
September 16, 2022
Li Chen, David K. Banfield
mono-HCl,Sigma,Cat#L5626
L-methionine
,
Sigma
,Cat#M9625
L-phenylalanine
,
Sigma
,Cat#P2126
L-serine
,
Sigma
,Cat#S4500 L-threonine,Sigma,Cat#T8625
L-tryptophan
,
Sigma
,Cat#T0254
L-tyrosine
,
Sigma
,Cat#T3754
L-valine
,
Sigma
,Cat#V0500
Uracil
,
Sigma
,Cat#U0750
MscI
restriction endonuclease,
New England Biolabs
,Cat#
R0534
Yeast Extract,Oxoid,Cat#LP0021 Peptone,Oxoid,Cat#LP0037
D-(+)- Glucose anhydrous
,RDH,Cat#16325
D-(+)- Galactose
(2)
Insight and Development of Advanced Recombinant Adeno-Associated Virus Analysis Tools Exploiting Single-Particle Quantification by Multidimensional Droplet Digital PCR
Human Gene Therapy
September 1, 2022
Jeanette Zanker, Sara Lázaro-Petri, Daniela Hüser, Regine Heilbronn, Adrien Savy
0.1 U/μL of
SmaI
(
New England Biolabs
—no. RS0141S) or with 0.1 U/μL of
MscI
(
New England Biolabs
—no.
R0534L
) following the manufacturer's instructions. A total of 4 × 1011 vector genomes of rAAV1 were treated
(3)
Establishment of centromere identity is dependent on nuclear spatial organization
Current Biology
July 25, 2022
Weifang Wu, Toni McHugh, David A. Kelly, Alison L. Pidoux, Robin C. Allshire
,
New England Biolabs
,Cat#
R0146S
SacI-HF
,
New England Biolabs
,Cat#
R3156S
MscI
,
New England Biolabs
,Cat#
R0534S
T4 DNA Ligase
,
New England Biolabs
,Cat#
M0202S
PMSF,MERCK,CAS#329-98-6
Yeast
Protease Inhibitor Cocktail,MERCK,P8215 IGEPAL
(4)
Combining electrophysiology and optogenetics for functional screening of pyramidal neurons in the mouse prefrontal cortex
STAR Protocols
June 1, 2021
Kenichiro Nagahama, Shuhei Fujino, Takaki Watanabe, Naofumi Uesaka, Masanobu Kano
Inc",CAT: # R0101
AgeI
,"
New England BioLabs
, Inc",CAT: # R0552
MSCI
,"
New England BioLabs
, Inc",CAT: #
R0534
rAPid Alkaline Phosphatase
,"
Roche
Molecular Systems, Inc",CAT: # 4898141001 ,, Biological samples,Biological
(5)
Targeted Enrichment of rRNA Gene Tandem Arrays for Ultra-Long Sequencing by Selective Restriction Endonuclease Digestion
Frontiers in Plant Science
April 28, 2021
Anastasia McKinlay, Dalen Fultz, Feng Wang, Craig S. Pikaard
BstZ17I-HF
(
NEB
#R3594),
SpeI-HF
(
NEB
#R3133),
BclI-HF
(
NEB
#R3160),
SnaBI
(
NEB
#
R0130
),
MscI
(
NEB
#
R0534
), and
PvuII-HF
(
NEB
#R3151) at 37◦ C overnight. The buffer was then removed and replaced with 500 µL
(6)
Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences
Plant Communications
March 8, 2021
Simon Sretenovic, Desuo Yin, Adam Levav, Jeremy D. Selengut, Stephen M. Mount, Yiping Qi
109328) (Lowder et al., 2015) containing z-SpyCas9 was enzymatically digested with
MscI
(
NEB
, catalog no.
R0534
∗) and
SacI
(
NEB
, catalog no. R3156∗). Based on the synthesized gBlocks and digested vectors, the NEBuilder
(7)
Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System
BIO-PROTOCOL
January 20, 2021
Laurie G Landry, Sarah E Mann, Amanda M Anderson, Maki Nakayama
(
NEB
, catalog number:
R0101S
) f.
XhoI
(
NEB
, catalog number:
R0146S
) g.
MscI
(
NEB
, catalog number:
R0534S
) h.
AleI-v2
(
NEB
, catalog number:
R0685S
) i.
SalI
(
NEB
, catalog number:
R0138S
) j.
BglII
(
NEB
,
(8)
A Non-Synonymous Single Nucleotide Polymorphism in
FASN
Gene Alters FASN Enzyme Activity in Subcutaneous and Intramuscular Adipose Tissue in Holstein Friesian Steers
Annals of Animal Science
January 1, 2021
David Cancino-Baier et al.
period at 72°C for 5 min. The 759 bp amplicon obtained was then digested by the restrictase
MscI
(Cat. #
R0534S
,
New England Biolabs
, Massachusetts, USA) which included
CutSmart buffer
1X,
MscI
restriction enzyme (3
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MscI from New England Biolabs
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